Joshua Prince, Duke Civil and Environmental Engineering and ITEHP PhD candidate, will present, "Understanding Charged Species Transport in Bacterial Biofilms via Modelling, Experiments and Simulations." It is estimated that worldwide, there are 100,000 people with cystic fibrosis (pwCF), a genetic-disease which reduces lung function and has historically led to significantly decreased healthspans. Chronic lung infections of Pseudomonas aeruginosa in pwCF are tied with worse health outcomes, and often present as biofilms, a mode of bacterial life which can confer antibiotic recalcitrance. One hypothesized mechanism for this recalcitrance is diffusion-limitations, which theorizes physical interactions between antibiotics and the biofilm extra-cellular matrix cause reduced antibiotic efficacy. Our research aims to investigate this mechanism by proposing new physical models which replicate existing experimental evidence for this hypothesis, performing our own experimental investigations in synthetic biofilm analogues, and running physical simulations to test model predictions in these experimental systems.